The Plant Pathology Journal

Fig. 1.

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Fig. 1. Non-invasive diagnosis of microbial pathogenic infection and diseased plant tissues by volatile analysis. Volatile interpretation can be separated into two phases: 1. Volatile collection and 2. Volatile analysis. Analysis techniques include quantification of relative amounts of different volatiles and real-time analysis to assess ongoing changes. Volatile collection tools include dynamic sampling methods such as closed-loop-stripping-analysis (CLSA) and static headspace sampling methods such as solid phase microextraction (SPME), stir bar sorptive extraction (SBSE), and silicone tubing (ST). Quantification methods involve conventional gas chromatography-mass spectrometry (GC-MS) and portable GC-MS devices. Real-time analysis tools include Proton Transfer Reaction (PTR)-MS, multi-capillary column (MCC)-PTR-MS and E-nose. Two of these technologies, E-nose and portable GC-MS, can be used in the field.
The Plant Pathology Journal 2018;34:459-69 https://doi.org/10.5423/PPJ.RW.06.2018.0118
© 2018 The Plant Pathology Journal