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Fig. 2.

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Fig. 2. Specificity assay for the designed primer pairs (Bglu3 and Bgla9), by amplification of the expected PCR products (A) Products from bacterial genomic DNA. (B) Products from cell suspensions. Lane 1, Burkholderia glumae BGR1; lane 2, B. gladioli BSR3; lane 3, B. cepacia KACC 10189; lane 4, B. cepacia KACC 10190; lane 5, B. cepacia KACC 10337; lane 6, B. cepacia KACC 12679; lane 7, B. cepacia KACC 15010; lane 8, B. kururiensis KACC 12038; lane 9, Burkholderia sp. KJ006; lane 10, B. megalochromosomata KACC 17925; lane 11, B. phymatum KACC 12032; lane 12, B. phytofirmans KACC 12042; lane 13, B. pyrrocinia KACC 17914; lane 14, B. stabilis KACC 12028. Specific bands were clearly visible on the 2% agarose gel at 174 bp for B. glumae BGR1 and 289 bp for B. gladioli BSR3. No bands were observed with the other tested Burkholderia species. M denotes the 1 kb DNA ladder.
The Plant Pathology Journal 2018;34:490-8
© 2018 The Plant Pathology Journal