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Fig. 3.

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Fig. 3. Sensitivity assay for the designed primer pairs (Bglu3 and Bgla9), by amplification of the expected PCR product from genomic DNA (gDNA) from (A) Burkholderia glumae BGR1, (B) B. gladioli BSR3 and (C) mixed sample (1:1) of B. glumae BGR1 and B. gladioli BSR3 in a duplex PCR assay Lane 1, 100 ng gDNA; lane 2, 10 ng gDNA; lane 3, 1 ng gDNA; lane 4, 0.1 ng gDNA; lane 5, 0.01 ng gDNA, lane 6, 1 pg gDNA, lane 7, 0.1 pg; lane 8, no gDNA template. Specific bands were clearly visible on the 2% agarose gel at 174 bp for B. glumae and 289 bp for B. gladioli up to the 0.1 ng gDNA template. M denotes the 1 kb DNA ladder.
The Plant Pathology Journal 2018;34:490-8 https://doi.org/10.5423/PPJ.OA.07.2018.0136
© 2018 The Plant Pathology Journal