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Fig. 4.

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Fig. 4. Sensitivity assay for the designed primer pairs (Bglu3 and Bgla9), by amplification of the expected PCR product from bacterial cell suspensions of (A) Burkholderia glumae BGR1, (B) Burkholderia gladioli BSR3 and (C) mixed sample of B. glumae BGR1 and B. gladioli BSR3 in the multiplex PCR assay Lane 1, Overnight cultures; lane 2–10, serially diluted cultures (101 to 109); lane 11, no cells. Clear specific bands were visible on the 2% agarose gel at 174 bp for B. glumae BGR1 and 289 bp for B. gladioli BSR3 up to the 103 dilution (approximately 300 cells for B. glumae and approximately 500 cells for B. gladioli). M denotes the 1 kb DNA ladder.
The Plant Pathology Journal 2018;34:490-8 https://doi.org/10.5423/PPJ.OA.07.2018.0136
© 2018 The Plant Pathology Journal