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Fig. 5.

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Fig. 5. Sensitivity assay for the designed primer pairs (Bglu3 and Bgla9), by amplification of the expected PCR product from rice seeds inoculated with different dilutions of Burkholderia glumae BGR1, Burkholderia gladioli BSR3 and mixed co-inoculation Suspensions from rice seeds inoculated with different dilutions of the tested strains or their mix were collected using sterile distilled water containing 0.03% Tween 20 and used as templates for the PCR. Lane 1, 100 ng gDNA from B. glumae BGR1; lane 2, 100 ng gDNA from B. gladioli BSR3; lane 3, cell suspension of overnight culture from B. glumae; lane, 4–7, 101 to 104 dilutions; lane 8, cell suspension of overnight culture from B. gladioli; lane 9–12, 101 to 104 dilutions; lane 13, 1:1 cell suspension mix from overnight culture from B. glumae and B. gladioli; lane 14–17, 101 to 104 dilutions. M, 1 kb DNA ladder; lane 18, un-inoculated surface sterilized rice seeds as a negative control.
The Plant Pathology Journal 2018;34:490-8 https://doi.org/10.5423/PPJ.OA.07.2018.0136
© 2018 The Plant Pathology Journal