The Plant Pathology Journal

Fig. 2.

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Fig. 2. Loop-mediated isothermal amplification (LAMP) for specific detection of ‘Candidatus Liberibacter asiaticus’ using the primer set from the prophage gene, LasAI in HLB-infected leaves of grapefruit according to reaction temperatures (57, 60, 62 and 65°C). condition test for HLB detection. (A) Visual detection under normal light by adding SYBR Green I dye. (B) Electrophoresis analysis on 1% agarose gel. Lanes 1–4; HLB-infected grapefruit leaves, lanes 5–8; healthy grapefruit leaves, lanes 9–11; distilled water, lane M; 100 bp DNA ladder (NEB New England Biolabs, cat# N3231S).
The Plant Pathology Journal 2018;34:499-505
© 2018 The Plant Pathology Journal