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Fig. 4.

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Fig. 4. Sensitivity of loop-mediated isothermal amplification (LAMP), conventional PCR and real-time PCR for detecting HLB. (A) Visual examination of LAMP products by adding SYBR Green I dye. (B) Electrophoresis analysis of LAMP products on 1% agarose gel. (C) Electrophoresis analysis of conventional PCR products on 1% agarose gel. (D) Sensitivity of real-time PCR for detecting HLB using primers (LJ900p, LJ900r)/probe (LJ900p) listed in . Tube and lane 1–8, and template DNA for real-time PCR; serially diluted genomic DNA (1, 10−1, 10−2, 10−3, 10−4, 10−5, 10−6) and distilled water as negative control, respectively, M; 100 bp DNA ladder.
The Plant Pathology Journal 2018;34:499-505 https://doi.org/10.5423/PPJ.FT.10.2018.0212
© 2018 The Plant Pathology Journal