The Plant Pathology Journal

Fig. 4.

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Fig. 4. Protective effects of Bacillus siamensis H30-3 against fungal pathogens on Chinese cabbage plants. (A) Dual culture assay for in vitro inhibition of mycelial growth of Alternaria brassicicola and Colletotrichum higginsianum by B. siamensis H30-3. The fungal pathogens were co-cultured with the bacterial strain H30-3 for 15 and 12 days at 25°C for A. brassicicola and C. higginsianum, respectively. (B) Inhibitory mycelial growth measured by half of the fungal colony diameter after co-culture. Error bars represent the standard errors of the means of the six independent experimental replications. Asterisks indicate significant differences as determined by Student’s t-test (P < 0.05). (C) Reduced black spot and anthracnose disease severities on Chinese cabbage plants by the antagonistic B. siamensis H30-3. Bacterial suspension (109 cfu/ml) of B. siamensis H30-3 was foliar sprayed at 1 day prior to challenge inoculations of the fungal pathogens. Disease severities were evaluated at 4 days after fungal inoculation based on 0–5 scales. Error bars represent the standard errors of the means of the four independent experimental replications. Means followed by the same letter are not significantly different at 5% level by least significant difference test.
The Plant Pathology Journal 2018;34:555-66
© 2018 The Plant Pathology Journal