The Plant Pathology Journal

Fig. 5.

Download original image
Fig. 5. Antifungal metabolites production and induced defence response of Chinese cabbage plants by Bacillus siamensis H30-3. (A) PCR-based detection of bacterial genes encoding antimicrobial lipopeptides from B. aryabhattai H26-2 and B. siamensis H30-3 genomes. M, DNA size marker. bacD, bacilysin; bmyA, bacillomycin; fenD, fengycin; ituA, iturin A; srfA, surfactin; zwiA, zwittermicin A. (B) Characteristics of hydrolytic enzyme secretion, siderophore production and phosphate (P)-solubilisation originated from B. siamensis H30-3. In vitro productions of chitinase, protease and cellulase by B. siamensis H30-3 were examined on different agar media described in Materials and methods. (C) Expression of defence-related genes in Chinese cabbage leaves treated with B. siamensis H30-3. Expression of basic glucanase 2 gene BrBGL2, chitinase 1 gene BrChi1, glutathione-S-transferase 1 gene BrGST1 and ascorbate peroxidase 1 gene BrAPX1 was analyzed by semi-quantitative RT-PCR technique. BrActin7 was used as an internal control. The number of PCR cycles of each result is indicated within the right parenthesis.
The Plant Pathology Journal 2018;34:555-66
© 2018 The Plant Pathology Journal