The Plant Pathology Journal

Fig. 1.

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Fig. 1. Purification of the PeBL2 protein. (A) Cation exchange chromatography using a HisTrap SP HP column (2.5 cm × 20 cm). The column was washed with buffer A (25 mM MES-NaOH, pH 6.2) to remove any unbound proteins, and the bound proteins were eluted with a linear gradient of increasing NaCl (0–1 M). Buffer B (25 mM Tris, 200 mM NaCl, 500 mM imidazole, pH 8.0) was used to B. Two fractions (peak a and peak b) were produced. (B) Fractions (peak a and peak b) along with BSA (control) included in the test for HR. Out of these fractions peak a showing HR in tobacco leaves. (C) SDS-PAGE of crude proteins.
Plant Pathol. J. 2019;35:208-18
© 2019 Plant Pathol. J.