The Plant Pathology Journal

Fig. 2.

Download original image
Fig. 2. Purification of the active fraction. (A) Waters Atlantis T3 C18 reversed-phase column (2.1 mm × 150 mm, 3.5 m, 40°C) was equilibrated with 5% CAN and acetonitrile/2 mM NH4FA/0.1% FA/water. The concentration of ACN in the eluted solvent was raised from 10% (v/v) to 60% (v/v) over 28 min using a linear gradient at a flow rate of 0.2 ml/min. The peaks a, b, c, d, e and f were produced by HPLC. (B) All peaks were checked for HR in tobacco leaves. Out of all peaks peak d displayed an obvious HR.
Plant Pathol. J. 2019;35:208-18
© 2019 Plant Pathol. J.