The Plant Pathology Journal

Fig. 1.

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Fig. 1. Yeast two hybrid (Y2H) assays for Burkholderia glumae PVF11 interaction with rice defense-related proteins. (A) Positive Y2H interaction between PVF11, as prey, and WRKY transcriptional factors, as baits. The empty bait vector was used as a negative control. (B) Positive Y2H interaction between PVF11, as bait, and rice cDNA libraries (Y28, Y29 and Y34) as prey. In both Y2H assays, positive interactions are indicated by hybridized yeast cell survival on LTH (SD/-His/-Leu/-Trp) and LTHA (SD/-Ade/-His/-Leu/-Trp) agar plates.
Plant Pathol. J. 2019;35:280-6 https://doi.org/10.5423/PPJ.NT.02.2019.0026
© 2019 Plant Pathol. J.