The Plant Pathology Journal

Fig. 5.

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Fig. 5. Spray timing of cell suspensions of S. padanus PMS-702 for controlling downy mildew severity on cucumber leaves. (A) Cucumber leaves showing downy mildew symptoms after treated with PMS-702 suspensions cultured in SMG-C-2 amended with Twee 80 or water (control). (B) Quantification of downy mildew severity on cucumber leaves. PMS-702 was cultured in soybean meal-glucose amended with 1% coconut oil and sticky substances (SMG-C-2), with or without 2% Tween 80 for 5 days and sprayed onto 3-week-old cucumber seedlings 24 h before inoculation (hbi), post inoculation (hpi) or co-application (0 hbi) with sporangial suspensions (104 sporangia/ml) of P. cubensis. Plants sprayed with water were used as mock controls. The treated plants were bagged for 2 days and maintained in a greenhouse after bag removal. Downy mildew severity was examined 7 dai. Data are means of three biological replicates. Means indicated by different letters were significantly different according to the Fisher’s least significance difference test (P = 0.05).
Plant Pathol. J. 2019;35:341-50 https://doi.org/10.5423/PPJ.OA.03.2019.0057
© 2019 Plant Pathol. J.