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Fig. 1.

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Fig. 1.

The phylogenetic tree of the FgSvf1 protein and FgSvf1 mutant construction. (A) The phylogenetic tree of FgSvf1 homologs in several fungal species is shown. The alignment was performed with ClustalW, and the MEGA program version 7.0 was used to perform a 1,000-bootstrap phylogenetic analysis using the neighbor joining method. (B) FgSvf1 targeted gene deletion, overexpression, and complementation. Deletion mutant (ΔFgSvf1) was generated by homologous gene recombination strategy through deleted FgSvf1 gene from the Fusarium graminearum (GZ3639) genomic DNA. Elongation factor 1 alpha promoter (EFpro) was used to generate FgSvf1 gene overexpression mutant (FgSvf1-OE) instead of native promoter of F. graminearum. The complementation strain (FgSvf1-C) was created using the ΔFgSvf1 strain. (C) Expression levels of FgSvf1 in ΔFgSvf1, FgSvf1-OE, FgSvf1-C. Relative expression of FgSvf1 was measured by quantitative real-time PCR. The bar denotes the standard error from three repeated experiments. HYG, hygromycin resistance cassette; GEN, geneticin resistance cassette.

Plant Pathol. J. 2019;35:393-405 https://doi.org/10.5423/PPJ.OA.03.2019.0070
© 2019 Plant Pathol. J.