The Plant Pathology Journal

Fig. 2.

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Fig. 2.

Influence of FgSvf1 on Fusarium graminearum morphology. (A) Influence of FgSvf1 on vegetative growth. GZ3639, ΔFgSvf1, FgSvf1-OE, and FgSvf1-C were grown on complete medium (CM), minimal medium (MM), potato dextrose agar (PDA), and yeast malt agar medium (YMA) at 25°C for 4 days. (B) Influence of FgSvf1 gene on conidia germination. Conidia were inoculated into potato dextrose broth, and the cultures were shaken at 200 rpm at 25°C. After 4, 8, and 12 h of incubation, the conidia were analyzed for germination. Asterisks indicate significant differences (*P < 0.05, **P < 0.01, ***P < 0.001) based on the Tukey’s test in the program R. (C) Involvement of FgSvf1 in sexual development. Each strain was inoculated onto carrot agar for 8 days, and then sexual reproduction was induced by placing the cultures under a near ultraviolet lamp at 25°C. After 10 days, self-fertilization was examined using a dissection microscope. Upper: black, ball-like structures are perithecia. Scale bar = 1 mm. Lower: asci rosettes of each strain were observed microscopically 10 days after sexual induction. Scale bar = 10 μm.

Plant Pathol. J. 2019;35:393-405
© 2019 Plant Pathol. J.