The Plant Pathology Journal

Fig. 4.

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Fig. 4.

Influence of FgSvf1 on resistance to the cold, a fungicide, osmotic stress, and sensitivity to oxidative stress. (A) Survival of GZ3639, ΔFgSvf1, FgSvf1-OE, and FgSvf1-C under cold stress. 103 conidia/ml of conidia were stored at 4°C for 1–4 days in distilled water. Then 100 μl of each suspension was spread onto potato dextrose agar (PDA) and the survived spore was measured after 3 days. The bar denotes the standard error from five experiments. (B) Resistance to the fungicide prochloraz. GZ3639, ΔFgSvf1, FgSvf1-OE, and FgSvf1-C were inoculated onto PDA containing 0.1–0.5 mg/l prochloraz for 7 days at 25°C. (C) Resistance to osmotic stress. GZ3639, ΔFgSvf1, FgSvf1-OE, and FgSvf1-C were inoculated onto PDA containing 0.2–1.8 M NaCl for 7 days at 25°C. The bar denotes the standard error from three experiments. (D) Sensitivity to oxidative stress. GZ3639, ΔFgSvf1, and FgSvf1-OE were inoculated onto PDA containing 0–5 mM H2O2 for 3 days at 25°C. Asterisks indicate significant differences (*P < 0.05, **P < 0.01, ***P < 0.001) based on the Tukey’s test in the program R.

Plant Pathol. J. 2019;35:393-405 https://doi.org/10.5423/PPJ.OA.03.2019.0070
© 2019 Plant Pathol. J.