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Fig. 7.

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Fig. 7.

Influence of FgSvf1 on phosphoenolpyruvate (PEP) production and expression of glycolytic genes. (A) PEP concentration in GZ3639, ΔFgSvf1, FgSvf1-OE, and FgSvf1-C. Mycelia were cultivated on potato dextrose agar (PDA) for 7 days, harvested, vacuum freeze-dried, and then PEP was extracted from 0.02 g of dried mycelia from each strain. All data were obtained from two biological replicates. Values with different letters are significantly different according to the Tukey’s test (P < 0.05) in the program R. (B) Normalized expression levels of genes coding enolase, pyruvate kinase, and the pyruvate dehydrogenase complex. RNA was extracted after cultivating each strain in PDA for 7 days. The bar denotes the standard error from three repeated experiments. Gene expression level were normalized to CYP gene expression. (C) Vegetative growth on medium containing sodium pyruvate. Three-day old cultures of GZ3639, ΔFgSvf1, FgSvf1-OE, and FgSvf1-C were cultivated on PDA containing 30 mM of sodium pyruvate for 7 days at 25°C.

Plant Pathol. J. 2019;35:393-405 https://doi.org/10.5423/PPJ.OA.03.2019.0070
© 2019 Plant Pathol. J.