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HR-Mediated Defense Response is Overcome at High Temperatures in Capsicum Species
Published online January 4, 2018
© 2018 The Korean Society of Plant Pathology.

Bong Nam Chung1*, Joung-Ho Lee2, Byoung-Cheorl Kang2, Sang Wook Koh3, Jae Ho Joa3, Kyung San Choi3, and Jeong Joon Ahn3

1National Institute of Horticultural & Herbal Science, RDA, Wanju 55365, Korea 2Department of Plant Science College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea 3Research Institute of Climate Change and Agriculture, Jeju 63240, Korea
Correspondence to: Phone) +82-63-238-6340, FAX) +82-63-238-6305
E-mail) chbn7567@korea.kr
Received June 10, 2017; Revised September 26, 2017; Accepted September 27, 2017.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http:// creativecommons.org/licenses/by-nc/4.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Resistance to Tomato spotted wilt virus isolated from paprika (TSWV-Pap) was overcome at high temperatures (30 ± 2°C) in both accessions of Capsicum annuum S3669 (Hana Seed Company) and C. chinense PI15225 (AVRDC Vegetable Genetic Resources). S3669 and PI15225, which carrying the Tsw gene, were mechanically inoculated with TSWV-Pap, and then maintained in growth chambers at temperatures ranging from 15 ± 2°C to 30 ± 2°C (in 5°C increments). Seven days post inoculation (dpi), a hypersensitivity reaction (HR) was induced in inoculated leaves of PI152225 and S3669 plants maintained at 25°C ± 2°C. Meanwhile, necrotic spots were formed in upper leaves of 33% of PI15225 plants maintained at 30 ± 2°C, while systemic mottle symptoms developed in 50% of S3669 plants inoculated. By 15 dpi, 25% of S3669 plants had recovered from systemic mottling induced at 30 ± 2°C. These results demonstrated that resistance to TSWV-Pap can be overcome at higher temperatures in both C. chinense and C. annuum. This is the first study reporting the determination of temperatures at which TSWV resistance is overcome in a C. annuum genetic resource expressing the Tsw gene. Our results indicated that TSWV resistance shown from pepper plants possess the Tsw gene could be overcome at high temperature. Thus, breeders should conduct evaluation of TSWV resistance in pepper cultivars at higher temperature than 30°C (constant temperature).
Keywords : HR, recovery, resistance, temperature, Tsw gene


December 2017, 33 (6)