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Genomic Insights into Nematicidal Activity of a Bacterial Endophyte, Raoultella ornithinolytica MG against Pine Wilt Nematode
Published online April 26, 2018
© 2018 The Korean Society of Plant Pathology.

Gnanendra Shanmugam1, Akanksha Dubey1, Lakshmi Narayanan Ponpandian1, Soon Ok Rim1, Sang-Tae Seo2, Hanhong Bae1*, and Junhyun Jeon1*

1Department of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 38541, Korea
2Division of Forest Insect Pests and Diseases, National Institute of Forest Science Seoul 02455, Korea
Correspondence to: H Bae
Phone) +82-53-810-3031, FAX) +82-53-810-4769
E-mail) hanhongbae@ynu.ac.kr
J Jeon
Phone) +82-53-810-3030, FAX) +82-53-810-4769
E-mail) jjeon@yu.ac.kr
Received October 20, 2017; Revised March 9, 2018; Accepted March 11, 2018.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Pine wilt disease, caused by the nematode Bursaphelenchus xylophilus, is one of the most devastating conifer diseases decimating several species of pine trees on a global scale. Here, we report the draft genome of Raoultella ornithinolytica MG, which is isolated from mountain-cultivated ginseng plant as an bacterial endophyte and shows nematicidal activity against B. xylophilus. Our analysis of R. ornithinolytica MG genome showed that it possesses many genes encoding potential nematicidal factors in addition to some secondary metabolite biosynthetic gene clusters that may contribute to the observed nematicidal activity of the strain. Furthermore, the genome was lacking key components of avermectin gene cluster, suggesting that nematicidal activity of the bacterium is not likely due to the famous anthelmintic agent of wide-spread use, avermectin. This genomic information of R. ornithinolytica will provide basis for identification and engineering of genes and their products toward control of pine wilt disease.
Keywords : bacterial endophyte, nematicidal activity, pine wilt disease (PWD)


April 2018, 34 (2)
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