search for




 

Transcriptomic Analysis of Oryza sativa Leaves Reveals Key Changes in Response to Magnaporthe oryzae MSP1
Plant Pathol. J. 2018;34:257-268
Published online August 1, 2018
© 2018 The Korean Society of Plant Pathology.

Qingfeng Meng1, Ravi Gupta1, Soon Jae Kwon1, Yiming Wang2, Ganesh Kumar Agrawal3, Randeep Rakwal3,4,5,6, Sang-Ryeol Park7, and Sun Tae Kim1*

1Department of Plant Bioscience, Life and Energy Convergence Research Institute, Pusan National University, Miryang 46241, Korea
2Department of Plant-Microbe Interactions, Max Planck Institute for Plant Breeding Research, Carl-von-Linne Weg 10, 50829 Cologne, Germany
3Research Laboratory for Biotechnology and Biochemistry (RLABB), GPO Box 13265, Kathmandu, Nepal
4GRADE Academy Private Limited, Adarsh Nagar-13, Birgunj, Nepal
5Faculty of Health and Sport Sciences and Tsukuba International Academy for Sport Studies (TIAS), University of Tsukuba, Ibaraki 305-8577, Japan
6Global Research Center for Innovative Life Science, Peptide Drug Innovation, School of Pharmacy and Pharmaceutical Sciences, Hoshi University, Tokyo 142-8501, Japan
7Gene Engineering Division, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, Korea
Correspondence to: Phone) +82-55-350-5505, FAX) +82-55-350-5509
E-mail) stkim71@pusan.ac.kr
Received January 23, 2018; Revised May 4, 2018; Accepted May 14, 2018.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Rice blast disease, caused by Magnaporthe oryzae, results in an extensive loss of rice productivity. Previously, we identified a novel M. oryzae secreted protein, termed MSP1 which causes cell death and pathogen-associated molecular pattern (PAMP)-triggered immune (PTI) responses in rice. Here, we report the transcriptome profile of MSP1-induced response in rice, which led to the identification of 21,619 genes, among which 4,386 showed significant changes (P < 0.05 and fold change > 2 or < 1/2) in response to exogenous MSP1 treatment. Functional annotation of differentially regulated genes showed that the suppressed genes were deeply associated with photosynthesis, secondary metabolism, lipid synthesis, and protein synthesis, while the induced genes were involved in lipid degradation, protein degradation, and signaling. Moreover, expression of genes encoding receptor-like kinases, MAPKs, WRKYs, hormone signaling proteins and pathogenesis-related (PR) proteins were also induced by MSP1. Mapping these differentially expressed genes onto various pathways revealed critical information about the MSP1-triggered responses, providing new insights into the molecular mechanism and components of MSP1-triggered PTI responses in rice.
Keywords : DNA microarray, Magnaporthe oryzae, MSP1, PAMP-triggered immunity, rice


August 2018, 34 (4)
  • DOAJ
  • ORCID