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Taxonomic and Functional Changes of Bacterial Communities in the Rhizosphere of Kimchi Cabbage After Seed Bacterization with Proteus vulgaris JBLS202
Plant Pathol. J. 2018;34:286-296
Published online August 1, 2018
© 2018 The Korean Society of Plant Pathology.

Dipto Bhattacharyya1†, Swarnalee Duta1†, Sang-Mi Yu2, Sang Chul Jeong2, and Yong Hoon Lee1,3*

1Division of Biotechnology, Chonbuk National University, Iksan 54596, Korea
2Freshwater Bioresources Utilization Division, Nakdonggang National Institute of Biological Resources, Sangju 37242, Korea
3Advanced Institute of Environment and Bioscience, Plant Medical Research Center, and Institute of Bio-industry, Chonbuk National University, Jeonju 54896, Korea
Correspondence to: Phone) +82-63-850-0841, FAX) +82-63-850-0834
E-mail) yonghoonlee@jbnu.ac.kr
ORCID http://orcid.org/0000-0001-9921-3871
Authors contributed equally for this study
Received March 20, 2018; Revised May 31, 2018; Accepted May 31, 2018.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http:// creativecommons.org/licenses/by-nc/4.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Maintenance of a beneficial microbial community, especially in the rhizosphere, is indispensable for plant growth and agricultural sustainability. In this sense, plant growth-promoting rhizobacteria (PGPR) have been extensively studied for their role in plant growth promotion and disease resistance. However, the impact of introducing PGPR strains into rhizosphere microbial communities is still underexplored. We previously found that the Proteus vulgaris JBLS202 strain (JBLS202) promoted growth of Kimchi cabbage and altered the relative abundance of total bacteria and Pseudomonas spp. in the treated rhizosphere. To extend these findings, we used pyrosequencing to analyze the changes in bacterial communities in the rhizosphere of Kimchi cabbage after introduction of JBLS202. The alterations were also evaluated by taxon-specific realtime PCR (qPCR). The pyrosequencing data revealed an increase in total bacteria abundance, including specific groups such as Proteobacteria, Acidobacteria, and Actinobacteria, in the treated rhizosphere. Time-course qPCR analysis confirmed the increase in the abundance of Acidobacteria, Actinobacteria, Alphaproteobacteria, and Betaproteobacteria. Furthermore, genes involved in nitrogen cycling were upregulated by JBLS202 treatment indicating changes in ecological function of the rhizosphere soil. Overall, these results indicate that introduction of JBLS202 alters both the composition and function of the rhizosphere bacterial community, which can have direct and indirect effects on plant growth. Therefore, we propose that long-term changes in bacterial composition and community-level function need to be considered for practical use of PGPRs.
Keywords : microbiome, PGPR, pyrosequencing, rhizosphere


August 2018, 34 (4)
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