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Morphological and Molecular Characterization of Heterodera schachtii and the Newly Recorded Cyst Nematode, H. trifolii Associated with Chinese Cabbage in Korea
Plant Pathol. J. 2018;34:297-307
Published online August 1, 2018
© 2018 The Korean Society of Plant Pathology.

Abraham Okki Mwamula1†, Hyoung-Rai Ko2†, Youngjoon Kim1, Young Ho Kim1, Jae-Kook Lee2, and Dong Woon Lee1*

1Department of Ecological Science, Kyungpook National University, Sangju 37224, Korea
2Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Korea
Correspondence to: Phone) +82-54-530-1212, FAX) +82-54-530-1218
E-mail) whitegrub@knu.ac.kr
ORCID http://orcid.org/0000-0001-9751-5390
These authors contributed equally to this work as first authors.
Received December 23, 2017; Revised March 6, 2018; Accepted March 13, 2018.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The sugar beet cyst nematode, Heterodera schachtii is a well known pathogen on Chinese cabbage in the highland fields of Korea. However, a race of cyst forming nematode with close morphological resemblance to H. trifolii was recently isolated from the same Chinese cabbage fields. Morphological species differentiation between the two cyst nematodes is challenging, with only minor differences between them. Thus, this study described the newly intercepted H. trifolii population, and reviewed morphological and molecular characteristics conceivably essential in differentiating the two nematode species. A comparison of morphometric measurements of both infective juveniles and vulval cones of cysts showed significant differences between the two cyst nematodes. When total RNA and genomic DNA were extracted from a mixed field population, COI genes and ITS regions were clearly amplified with primers of the two Heterodera species, suggesting that Heterodera population collected from the Chinese cabbage field consisted of a mixture of two species. COI and ITS of H. trifolii were predominantly amplified from nucleotides prepared from H. trifolii monoxenic population whereas those of H. schachtii were strongly detected in H. schachtii monoxenic cultures. Thus, this study confirms the coexistence of the two species in some Chinese cabbage fields; and the presence of H. trifolii in Korea is reported here for the first time.
Keywords : infective juvenile, morphometrics, vulval cone


August 2018, 34 (4)
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