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Simple Detection of Cochliobolus Fungal Pathogens in Maize
Plant Pathol. J. 2018;34:327-334
Published online August 1, 2018
© 2018 The Korean Society of Plant Pathology.

In Jeong Kang, Hyeong Kwon Shim, Jae Hwan Roh, Sunggi Heu, and Dong Bum Shin*

Crop Cultivation and Environment Research Division, National Institute of Crop Science, Suwon 16613, Korea
Correspondence to: Phone) +82-31-695-0653, FAX) +82-31-695-0635
E-mail) shindb@korea.kr
Received October 10, 2017; Revised May 30, 2018; Accepted June 29, 2018.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Northern corn leaf spot and southern corn leaf blight caused by Cochliobolus carbonum (anamorph, Bipolaris zeicola) and Cochliobolus heterostrophus (anamorph, Bipolaris maydis), respectively, are common maize diseases in Korea. Accurate detection of plant pathogens is necessary for effective disease management. Based on the polyketide synthase gene (PKS) of Cochliobolus carbonum and the nonribosomal peptide synthetase gene (NRPS) of Cochliobolus heterostrophus, primer pairs were designed for PCR to simultaneously detect the two fungal pathogens and were specific and sensitive enough to be used for duplex PCR analysis. This duplex PCR-based method was found to be effective for diagnosing simultaneous infections from the two Cochliobolus species that display similar morphological and mycological characteristics. With this method, it is possible to prevent infections in maize by detecting infected seeds or maize and discarding them. Besides saving time and effort, early diagnosis can help to prevent infections, establish comprehensive management systems, and secure healthy seeds.
Keywords : fungal diseases, maize, simultaneous diagnosis


August 2018, 34 (4)
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