search for


Disinfection by Ozone Microbubbles Can Cause Morphological Change of Fusarium oxysporum f. sp. melonis Spores
Plant Pathol. J. 2018;34:335-340
Published online August 1, 2018
© 2018 The Korean Society of Plant Pathology.

Masahiko Tamaki1* , Fumiyuki Kobayashi2, Hiromi Ikeura3, and Michio Sato1

1School of Agriculture, Meiji University, Kawasaki, Kanagawa 214-8571, Japan
2Faculty of Applied Life Science, Nippon Veterinary and Life Science University, Musashino, Tokyo 180-8602, Japan
3Faculty of Life and Environmental Science, Shimane University, Matsue, Shimane 690-8504, Japan
Correspondence to: Phone) +81-44-980-5276, FAX) +81-44-980-5276
Received November 12, 2017; Revised March 21, 2018; Accepted April 23, 2018.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
To investigate the difference in the disinfectant efficiency of ozone microbubbles (O3MB) and ozone millibubbles (O3MMB), the morphological change of the treated Fusarium oxysporum f. sp. melonis spores was observed with scanning and transmission electron microscopies (SEM and TEM). The disinfectant efficiency of O3MB on F. oxysporum f. sp. melonis spores was greater than that of O3MMB. On observation with SEM, it was revealed that morphological change of F. oxysporum f. sp. melonis spores was caused by O3MB and O3MMB, and damage to the spore surfaces by O3MB occurred sooner than that by O3MMB. On observation with TEM, it was furthermore confirmed that F. oxysporum f. sp. melonis spores treated with O3MB induced wavy deformation of cell membrane and the intracellular change different from that with O3MMB. Therefore, the greater disinfection efficiency of O3MB was suggested to be caused due to the function of the MB in addition to the oxidative power of O3.
Keywords : disinfection, Fusarium oxysporum f. sp. melonis spores, ozone microbubbles

August 2018, 34 (4)
  • DOAJ