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Comparative Pathogenicity of Fusarium graminearum Isolates from Wheat Kernels in Korea
Plant Pathol. J. 2018;34:347-355
Published online October 1, 2018
© 2018 The Korean Society of Plant Pathology.

Sanghyun Shin1†, Jae-Han Son1, Jong-Chul Park1, Kyeong-Hoon Kim1, Young-mi Yoon1, Young-Keun Cheong1, Kyong-Ho Kim1, Jong-Nae Hyun1, Chul Soo Park2, Ruth Dill-Macky3, and Chon-Sik Kang1*

1National Institute of Crop Science, RDA, Wanju 55365, Korea
2Department of Crop Science and Biotechnology, Jeonbuk National University, Jeonju 54896, Korea
3Department of Plant Pathology, University of Minnesota, 495 Borlaug Hall, 1991 Upper Buford Circle, St. Paul, MN 55108, USA
Present address: Animal and Plant Quarantine Agency, 167 Yongjeon-ro, Gimcheon 39660, Korea
Correspondence to: Phone) +82-63-238-5225, FAX) +82-63-238-5205
E-mail) kcs1209@korea.kr
Received February 1, 2018; Revised May 5, 2018; Accepted May 25, 2018.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Fusarium head blight (FHB) caused by Fusarium species is a major disease of wheat and barley around the world. FHB causes yield reductions and contamination of grains with trichothecene mycotoxins including; nivalenol (NIV), deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-ADON), and 15-acetyldeoxynivalenol (15-ADON). The objectives of this study were to identify strains of F. graminearum isolated in Korea from 2012-harvested wheat grain and to test the pathogenicity of these NIV- and DON-producing isolates. Three hundred and four samples of wheat grain, harvested in 2012 in Chungnam, Chungbuk, Gyeongnam, Jeonbuk, Jeonnam, and Gangwon provinces were collected. We recovered 44 isolates from the 304 samples, based on the PCR amplification of internal transcribed spacer (ITS) rRNA region and sequencing. Our findings indicate that F. asiaticum was the predominant (95% of all isolates) species in Korea. We recovered both F. asiaticum and F. graminearum from samples collected in Chungnam province. Of the 44 isolates recovered, 36 isolates had a NIV genotype while 8 isolates belonged to the DON genotype (3-ADON and 15-ADON). In order to characterize the pathogenicity of the strains collected, disease severity was assessed visually on various greenhouse-grown wheat cultivars inoculated using both NIV- and DON-producing isolates. Our results suggest that Korean F. graminearum isolates from wheat belong to F. asiaticum producing NIV, and both F. graminearum and F. asiaticum are not significantly different on virulence in wheat cultivars.
Keywords : deoxynivalenol (DON), Fusarium graminearum, nivalenol (NIV)


October 2018, 34 (5)
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