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Isolation and Identification of Fungal Species from the Insect Pest Tribolium castaneum in Rice Processing Complexes in Korea
Plant Pathol. J. 2018;34:356-366
Published online October 1, 2018
© 2018 The Korean Society of Plant Pathology.

Tae-Seong Yun1,2†, Sook-Young Park1†, Jihyun Yu1, Yujin Hwang1, and Ki-Jeong Hong1*

1Department of Plant Medicine, College of Life Science and Natural Resources, Sunchon National University, Suncheon 57922, Korea
2Pyeongtaek District Office, Animal and Plant Quarantine Agency, Pyeongtaek 17962, Korea
Correspondence to: Phone) +82-61-750-3867, FAX) +82-61-750-3208
E-mail) curcul@scnu.ac.kr
These authors contributed equally to this work.
Received February 13, 2018; Revised June 2, 2018; Accepted July 2, 2018.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The red flour beetle, Tribolium castaneum, is one of the most common and economically important pests of stored cereal products worldwide. Furthermore, these beetles can act as vectors for several fungal post-harvest diseases. In this study, we collected T. castaneum from 49 rice processing complexes (RPCs) nationwide during 2016-2017 and identified contaminating fungal species on the surface of the beetles. Five beetles from each region were placed on potato dextrose agar media or Fusarium selection media after wet processing with 100% relative humidity at 27°C for one week. A total of 142 fungal isolates were thus collected. By sequence analysis of the internal transcribed spacer region, 23 fungal genera including one unidentified taxon were found to be associated with T. castaneum. The genus Aspergillus spp. (28.9%) was the most frequently present, followed by Cladosporium spp. (12.0%), Hyphopichia burtonii (9.2%), Penicillium spp. (8.5%), Mucor spp. (6.3%), Rhizopus spp. (5.6%), Cephaliophora spp. (3.5%), Alternaria alternata (2.8%) and Monascus sp. (2.8%). Less commonly identified were genera Fusarium, Nigrospora, Beauveria, Chaetomium, Coprinellus, Irpex, Lichtheimia, Trichoderma, Byssochlamys, Cochliobolus, Cunninghamella, Mortierella, Polyporales, Rhizomucor and Talaromyces. Among the isolates, two known mycotoxin-producing fungi, Aspergillus flavus and Fusarium spp. were also identified. This result is consistent with previous studies that surveyed fungal and mycotoxin contamination in rice from RPCs. Our study indicates that the storage pest, T. castaneum, would play an important role in spreading fungal contaminants and consequently increasing mycotoxin contamination in stored rice.
Keywords : fungi, mycotoxin, rice processing complexes, Tribolium castaneum


October 2018, 34 (5)
  • DOAJ
  • ORCID