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Characterization of Sclerotinia sclerotiorum, an Emerging Fungal Pathogen Causing Blight in Hyacinth Bean (Lablab purpureus)
Plant Pathol. J. 2018;34:367-380
Published online October 1, 2018
© 2018 The Korean Society of Plant Pathology.

Ananya Prova1, Abdul Mannan Akanda2, Shaikhul Islam2, and Md. Motaher Hossain2*

1Department of Plant Pathology, EXIM Bank Agricultural University, Chapainawabganj-6300, Bangladesh
2Department of Plant Pathology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh
Correspondence to: Phone) +88-02-9205310, FAX) +88-02-9205316
E-mail) hossainmm@bsmrau.edu.bd
Received March 11, 2018; Revised July 17, 2018; Accepted July 22, 2018.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Stems and pods of hyacinth bean cultivated in a farmer’s field in Gazipur District, Bangladesh, were found rotted in nearly 5% hyacinth bean plants. A fungus having fluffy mycelium and large sclerotia was isolated from affected tissues. Combined results of morphological, molecular and pathological analyses identified the fungus as Sclerotinia sclerotiorum (Lib) de Bary. Inoculating the fungus on healthy hyacinth bean plants and pods reproduced the symptoms previously observed in the field. The three isolates obtained from naturally infected plants were cross inoculated in hyacinth bean, okra and African-American marigold and they were pathogenic to these hosts. The optimum temperature and pH for its growth were 20°C and pH 5.0, respectively. Sclerotial development was favored at pH 5.0. Sucrose and mannitol were the best carbon sources to support hyphal growth, while glucose was the most favourable for sclerotial development. The hyacinth bean genotypes, HB-82 (Rupban Sheem) and HB-102 were found highly resistant, while HB-94 (Ashina) was moderate resistant to the fungus. Finally, S. sclerotiorum was sensitive to Bavistin, Dithane M-45 and Rovral fungicides and Ca in the form of CaCl2. This observation could possibly aid in eliminating field loss in hyacinth bean caused by an emerging pathogenic fungus S. sclerotiorum.
Keywords : Fungicides, host resistance, mycelial growth, new pathogen, sclerotial development


October 2018, 34 (5)
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