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Characterization of the Lytic Bacteriophage phiEaP-8 Effective against Both Erwinia amylovora and Erwinia pyrifoliae Causing Severe Diseases in Apple and Pear
Plant Pathol. J. 2018;34:445-450
Published online October 1, 2018
© 2018 The Korean Society of Plant Pathology.

Jungkum Park1, Gyu Min Lee2, Donghyuk Kim3, Duck Hwan Park4, and Chang-Sik Oh1*

1Department of Horticultural Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104, Korea
2Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104, Korea
3School of Energy and Chemical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Korea
4Applied Biology Program, Division of Bioresource Sciences, Kangwon National University, Chuncheon 24341, Korea
Correspondence to: Phone) +82-31-201-2678, FAX) +82-31-204-8116
E-mail) co35@khu.ac.kr
ORCID
Chang-Sik Oh
http://orcid.org/0000-0002-2123-862X
Received June 8, 2018; Revised July 20, 2018; Accepted July 25, 2018.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Bacteriophages, bacteria-infecting viruses, have been recently reconsidered as a biological control tool for preventing bacterial pathogens. Erwinia amylovora and E. pyrifoliae cause fire blight and black shoot blight disease in apple and pear, respectively. In this study, the bacteriophage phiEaP-8 was isolated from apple orchard soil and could efficiently and specifically kill both E. amylovora and E. pyrifoliae. This bacteriophage belongs to the Podoviridae family. Whole genome analysis revealed that phiEaP-8 carries a 75,929 bp genomic DNA with 78 coding sequences and 5 tRNA genes. Genome comparison showed that phiEaP-8 has only 85% identity to known bacteriophages at the DNA level. PhiEaP-8 retained lytic activity up to 50°C, within a pH range from 5 to 10, and under 365 nm UV light. Based on these characteristics, the bacteriophage phiEaP-8 is novel and carries potential to control both E. amylovora and E. pyrifoliae in apple and pear.
Keywords : Bacteriophage, black shoot blight, Erwinia amylovora, Erwinia pyrifoliae, fire blight


October 2018, 34 (5)
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