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Complete Genome Sequence Analysis of Two Divergent Groups of Sweet potato chlorotic fleck virus Isolates Collected from Korea
Plant Pathol. J. 2018;34:451-457
Published online October 1, 2018
© 2018 The Korean Society of Plant Pathology.

Hae-Ryun Kwak1, Jaedeok Kim1, Mikyeong Kim1, Jang-Kyun Seo2, Jeong-Soo Kim3, and Hong-Soo Choi1*

1Crop Protection Division, National Institute of Agricultural Science, Wanju 55365, Korea
2Graduate school of International Agricultural Technology, Seoul National University, Pyeongchang 25354, Korea
3Department of Plant Medicine, Andong National University, Andong 36729, Korea
Correspondence to: Phone) +82-31-290-0430, FAX) +82-31-290-0434
E-mail) hschoi@korea.kr
Received March 11, 2018; Revised June 10, 2018; Accepted July 29, 2018.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The Sweet potato chlorotic fleck virus (SPCFV), of the genus Carlavirus (family Betaflexiviridae), was first detected as one of several viruses infecting sweet potatoes (Ipomea batatas L.) in Korea. Out of 154 sweet potato samples collected in 2012 that were showing virus-like symptoms, 47 (31%) were infected with SPCFV, along with other viruses. The complete genome sequences of four SPCFV isolates were determined and analyzed using previously reported genome sequences. The complete genomes were found to contain 9,104-9,108 nucleotides, excluding the poly-A tail, containing six putative open reading frames (ORFs). Further, the SPCFV Korean isolates were divided into two groups (Group I and Group II) by phylogenetic analysis based on the complete nucleotide sequences; Group I and Group II had low nucleotide sequence identities of about 73%. For the first time, we determined the complete genome sequence for the Group II SPCFV isolates. The amino acid sequence identity in coat proteins (CP) between the two groups was over 90%, whereas the amino acid sequence identity in other proteins was less than 80%. In addition, SPCFV Korean isolates had a low amino acid sequence identity (61% CPs and 47% in the nucleotide-binding protein [NaBp] region) to that of Melon yellowing-associated virus (MYaV), a typical Carlavirus.
Keywords : Complete genome sequences, phylogenetic analyses, sweet potato chlorotic fleck virus


October 2018, 34 (5)
  • DOAJ
  • ORCID