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Hydrogen Peroxide Prompted Lignification Affects Pathogenicity of Hemi-biotrophic Pathogen Bipolaris sorokiniana to Wheat
Plant Pathol. J. 2019;35:287-300
Published online August 1, 2019
© 2019 The Korean Society of Plant Pathology.

Ajit Poudel1, Sudhir Navathe1,5 , Ramesh Chand1* , Vinod K. Mishra1, Pawan K. Singh2, and Arun K. Joshi3,4

1Institute of Agricultural Sciences, Banaras Hindu University Varanasi- 221005 UP, India
2International Maize and Wheat Improvement Center (CIMMYT), Apdo Postal 6-641, Mexico DF Mexico
3Borlaug Institute for South Asia (BISA), G-2, B-Block, NASC Complex, DPS Marg, New Delhi – 110012 India
4International Maize and Wheat Improvement Center (CIMMYT), G-2, B-Block, NASC Complex, DPS Marg, New Delhi – 110012 India
5Current Address: Agharkar Research Institute, G.G. Agarkar Road, Pune-411004, India
Correspondence to: Phone) +91-9415992810
E-mail) rc_vns@yahoo.co.in
ORCID
Sudhir Navathe
https://orcid.org/0000-0002-8040-5563
Ramesh Chand
https://orcid.org/0000-0001-5231-224X
Received September 7, 2018; Revised April 9, 2019; Accepted April 22, 2019.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Spot blotch caused by Bipolaris sorokiniana has spread to more than 9 million ha of wheat in the warm, humid areas of the Eastern Gangetic Plains (EGP) of South Asia and is a disease of major concern in other similar wheat growing regions worldwide. Differential lignin content in resistant and susceptible genotypes and its association with free radicals such as hydrogen peroxide (H2O2), superoxide (O2-) and hydroxyl radical (OH-) were studied after inoculation under field conditions for two consecutive years. H2O2 significantly influenced lignin content in flag leaves, whereas there was a negative correlation among lignin and H2O2 to the Area Under Disease Progress Curve (AUDPC). The production of H2O2 was higher in the resistant genotypes than susceptible ones. The O2- and OH- positively correlated with AUDPC but negatively with lignin content. This study illustrates that H2O2 has a vital role in prompting lignification and thereby resistance to spot blotch in wheat. We used cluster analysis to separate the resistant and susceptible genotypes by phenotypic and biochemical traits. H2O2 associated lignin production significantly reduced the number of appressoria and penetration pegs. We visualized the effect of lignin in disease resistance using differential histochemical staining of tissue from resistant and susceptible genotypes, which shows the variable accumulation of hydrogen peroxide and lignin around penetration sites.
Keywords : lignin, reactive oxygen species, resistance, spot blotch


August 2019, 35 (4)
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