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Identification of Proteomic Components Associated with Resistance to Fusarium Head Blight in Rye
Plant Pathol. J. 2019;35:313-320
Published online August 1, 2019
© 2019 The Korean Society of Plant Pathology.

Dawid Perlikowski1, Halina Wiśniewska1, Tomasz Góral2, Piotr Ochodzki2, Maciej Majka1, Izabela Pawłowicz1, Jolanta Belter1, and Arkadiusz Kosmala1*

1Institute of Plant Genetics, Polish Academy of Sciences, 60-479 Poznan, Poland
2Plant Breeding and Acclimatization Institute – National Research Institute, 05-870 Blonie, Poland
Correspondence to: Phone) +48 (61) 6550 285, FAX) +48 (61) 6550 301
E-mail) akos@igr.poznan.pl
ORCID
Arkadiusz Kosmala
https://orcid.org/0000-0002-1760-1334
Received November 29, 2018; Revised April 1, 2019; Accepted April 9, 2019.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Rye was used here to dissect molecular mechanisms of resistance to Fusarium head blight (FHB) and to go deeper with our understanding of that process in cereals. F. culmorum-damaged kernels of two lines different in their potential of resistance to FHB were analyzed using two-dimensional gel electrophoresis and mass spectrometry to identify resistance markers. The proteome profiling was accompanied by measurements of α- and β-amylase activities and mycotoxin content. The proteomic studies indicated a total of 18 spots with clear differences in protein abundance between the more resistant and more susceptible rye lines after infection. Eight proteins were involved in carbohydrate metabolism of which six proteins showed a significantly higher abundance in the resistant line. The other proteins recognized here were involved in stress response and redox homeostasis. Three remaining proteins were associated with protease inhibition/resistance and lignin biosynthesis, revealing higher accumulation levels in the susceptible rye line. After inoculation, the activities of α- and β-amylases, higher in the susceptible line, were probably responsible for a higher level of starch decomposition after infection and a higher susceptibility to FHB. The presented results could be a good reference for further research to improve crop resistance to FHB.
Keywords : FHB, Fusarium, proteome, resistance, rye


August 2019, 35 (4)
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