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Pathogenic Diversity of Ascochyta rabiei Isolates and Identification of Resistance Sources in Core Collection of Chickpea Germplasm
Plant Pathol. J. 2019;35:321-329
Published online August 1, 2019
© 2019 The Korean Society of Plant Pathology.

Somayeh Farahani1, Reza Talebi2* , Mojdeh Maleki1*, Rahim Mehrabi3, and Homayoun Kanouni4

1Department of Plant Protection, Varamin-Pishva Branch, Islamic Azad University, Varamin 3381774895, Iran
2Department of Agronomy & Plant Breeding, College of Agriculture, Sanandaj Branch, Islamic Azad University, Sanandaj 618, Iran
3Department of Biotechnology, College of Agriculture, Isfahan University of Technology, Isfahan 8415683111, Iran
4Kordestan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Sanandaj 33623351, Iran
Correspondence to: Reza Talebi
Phone) +98-8733367112, FAX) +98-8733367110
Mojdeh Maleki
Phone) +98-2136220650, FAX) +98-2136724767
Received December 23, 2018; Revised April 26, 2019; Accepted May 17, 2019.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Ascochyta blight caused by Ascochyta rabiei (Pass.) Lab. (Telomorph: Didymella rabiei) (Kov.) is one of the most important fungal diseases in chickpea worldwide. Knowledge about pathogen aggressiveness and identification resistance sources to different pathotypes is very useful for proper decisions in breeding programs. In this study, virulence of 32 A. rabiei isolates from different part of Iran were analyzed on seven chickpea differentials and grouped into six races based on 0-9 rating scale and susceptibility/resistant pattern of chickpea differentials. The least and most frequent races were race V and race I, respectively. Race V and VI showed highly virulence on most of differential, while race I showed least aggressiveness. Resistance pattern of 165 chickpea genotypes also were tested against six different A. rabiei races. ANOVA analysis showed high significant difference for isolate, chickpea genotypes and their interactions. Overall chickpea × isolate (race) interactions, 259 resistance responses (disease severity ≤ 4) were identified. Resistance spectra of chickpea genotypes showed more resistance rate to race I (49.70%) and race III (35.15%), while there were no resistance genotypes to race VI. Cluster analysis based on disease severity rate, grouped chickpea genotypes into four distinct clusters. Interactions between isolates or races used in this study, showed the lack of a genotype with complete resistance. Our finding for virulence pattern of A. rabiei and newly identified resistance sources could be considerably important for integration of ascochyta blight resistance genes into chickpea breeding programs and proper decision in future for germplasm conservation and diseases management.
Keywords : Ascochyta blight, chickpea, pathogenic variability, resistance sources

August 2019, 35 (4)
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