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An in vitro Actinidia Bioassay to Evaluate the Resistance to Pseudomonas syringae pv. actinidiae
Plant Pathol. J. 2019;35:372-380
Published online August 1, 2019
© 2019 The Korean Society of Plant Pathology.

Faming Wang, Jiewei Li, Kaiyu Ye, Pingping Liu, Hongjuan Gong, Qiaosheng Jiang, Beibei Qi, and Quanhui Mo*

Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guangxi Institute of Botany, Guilin 541006, China
Correspondence to: Phone) (+86)773-3550103, FAX) (+86)773-3550067
E-mail) quanhmoon@163.com
Received February 20, 2019; Revised May 15, 2019; Accepted May 19, 2019.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Pseudomonas syringae pv. actinidiae (Psa) is by far the most important pathogen of kiwifruit. Sustainable expansion of the kiwifruit industry requires the use of Psa-tolerant or resistant genotypes for the breeding of tolerant cultivars. However, the resistance of most existing kiwifruit cultivars and wild genotypes is poorly understood, and suitable evaluation methods of Psa resistance in Actinidia have not been established. A unique in vitro method to evaluate Psa resistance has been developed with 18 selected Actinidia genotypes. The assay involved debarking and measuring the lesions of cane pieces inoculated with the bacterium in combination with the observation of symptoms such as callus formation, sprouting of buds, and the extent to which Psa invaded xylem. Relative Psa resistance or tolerance was divided into four categories. The division results were consistent with field observations. This is the first report of an in vitro assay capable of large-scale screening of Psa-resistance in Actinidia germplasm with high accuracy and reproducibility. The assay would considerably facilitate the breeding of Psa-resistant cultivars and provide a valuable reference and inspiration for the resistance evaluation of other plants to different pathogens.
Keywords : canker disease, in vitro bioassay, kiwifruit, Pseudomonas syringae pv. actinidiae, resistance


August 2019, 35 (4)
  • DOAJ
  • ORCID