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Five Newly Collected Turnip Mosaic Virus (TuMV) Isolates from Jeju Island, Korea are Closely Related to Previously Reported Korean TuMV Isolates but Show Distinctive Symptom Development
Plant Pathol. J. 2019;35:381-388
Published online August 1, 2019
© 2019 The Korean Society of Plant Pathology.

Wen-Xing Hu1†, Byoung-Jo Kim1†, Younghwan Kwak1†, Eun-Young Seo1, Jung-Kyu Kim1, Jae-Yeong Han1,
Ik-Hyun Kim1, Yong Pyo Lim2, In-Sook Cho3, Leslie L Domier4, John Hammond5* , and Hyoun-Sub Lim1*

1Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Korea
2Molecular Genetics and Genomics Laboratory, Department of Horticulture, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Korea
3National Institute of Horticultural & Herbal Science, Rural Development Administration, Wanju 55365, Korea
4Soybean/Maize Germplasm, Pathology, and Genetics Research Unit, United States Department of Agriculture-Agricultural Research Service, Urbana, IL 61801, USA
5Floral and Nursery Plants Research Unit, United States National Arboretum, United States Department of Agriculture-Agricultural Research Service, Beltsville, MD 20705, USA
Correspondence to: Hyoun-Sub Lim
Phone) +82 42-821-5766, FAX) +82-42-823-8679
E-mail) hyounlim@cnu.ac.kr
John.Hammond
Phone) +1 301-504-5313, FAX) +1 301-504-5096
E-mail) john.hammond@ars.usda.gov
ORCID
https://orcid.org/0000-0001-7593-747X

These authors contributed equally to this study.
Received November 1, 2018; Revised April 29, 2019; Accepted May 21, 2019.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
For several years, temperatures in the Korean peninsula have gradually increased due to climate change, resulting in a changing environment for growth of crops and vegetables. An associated consequence is that emerging species of insect vector have caused increased viral transmission. In Jeju Island, Korea, occurrences of viral disease have increased. Here, we report characterization of five newly collected turnip mosaic virus (TuMV) isolates named KBJ1, KBJ2, KBJ3, KBJ4 and KBJ5 from a survey on Jeju Island in 2017. Full-length cDNAs of each isolate were cloned into the pJY vector downstream of cauliflower mosaic virus 35S and bacteriophage T7 RNA polymerase promoters. Their full-length sequences share 98.9-99.9% nucleotide sequence identity and were most closely related to previously reported Korean TuMV isolates. All isolates belonged to the BR group and infected both Chinese cabbage and radish. Four isolates induced very mild symptoms in Nicotiana benthamiana but KBJ5 induced a hypersensitive response. Symptom differences may result from three amino acid differences uniquely present in KBJ5; Gly(382)Asp, Ile(891)Val, and Lys(2522)Glu in P1, P3, and NIb, respectively.
Keywords : hypersensitive response, mild symptoms, turnip mosaic virus


August 2019, 35 (4)
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