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A Biostimulant Preparation of Brown Seaweed Ascophyllum nodosum Suppresses Powdery Mildew of Strawberry
Plant Pathol. J. 2019;35:406-416
Published online October 1, 2019
© 2019 The Korean Society of Plant Pathology.

Sruti Bajpai1, Pushp Sheel Shukla1, Samuel Asiedu2, Kris Pruski2, and Balakrishnan Prithiviraj1*

1Marine Bio-products Research Laboratory, Department of Plant, Food and Environmental Sciences, Dalhousie University, Truro, NS B2N 5E3, Canada
2Department of Plant, Food and Environmental Sciences, Dalhousie University, Truro, NS B2N 5E3, Canada
Correspondence to: Phone) +1-902-893-6036, FAX) +1-902-893-1404
Balakrishnan Prithiviraj

Handling Editor : Park, Chang-Jin
Received March 26, 2019; Revised May 17, 2019; Accepted May 21, 2019.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Strawberry, an important fruit crop, is susceptible to a large number of pathogens that reduce fruit quality and productivity. In this study, the effect of a biostimulant prepared from Ascophyllum nodosum extract (ANE) (0.1%, 0.2%, and 0.3%) was evaluated on powdery mildew progression under greenhouse and field conditions. In the greenhouse, application of 0.2% ANE showed maximum reduction in powdery mildew progression as compared to the control. Forty-eight hour post-inoculation, foliar spray of 0.2% ANE reduced spore germination by 75%. Strawberry leaves sprayed with ANE showed higher total phenolic and flavonoid content in response to powdery mildew infection. Furthermore, application of ANE elicited defense response in strawberry plants by induction of defense-related enzymes, such as phenylalanine ammonia lyase, polyphenol oxidase, and peroxidase activity. In field conditions, foliar spray of 0.2% ANE showed a reduction of 37.2% of natural incidence of powdery mildew infection as compared to the control. ANE sprayed plant also reduces the severity of powdery mildew infection under natural conditions. These results indicate that application of ANE induces the strawberry plant's active defense against powdery mildew infection by induction of secondary metabolism and regulating the activities of defense-related enzymes.
Keywords : Ascophyllum nodosum extract, phenylalanine ammonia lyase, Podosphaera aphanis, powdery mildew, strawberry

October 2019, 35 (5)
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