search for




 

Stress Tolerance and Virulence-Related Roles of Lipopolysaccharide in Burkholderia glumae
Plant Pathol. J. 2019;35:445-458
Published online October 1, 2019
© 2019 The Korean Society of Plant Pathology.

Chaeyeong Lee1,2†, Mohamed Mannaa1,2†, Namgyu Kim1,2, Juyun Kim1,2, Yeounju Choi1,2, Soo Hyun Kim1,2, Boknam Jung3, Hyun-Hee Lee1,2, Jungkwan Lee3, and Young-Su Seo1,2*

1Department of Microbiology, Pusan National University, Busan 46241, Korea
2Department of Integrated Biological Science, Pusan National University, Busan 46241, Korea
3Department of Applied Biology, Dong-A University, Busan 49315, Korea
Correspondence to: Phone) +82-51-510-2267, FAX) +82-51-514-1778
E-mail) yseo2011@pusan.ac.kr
ORCID
Young-Su Seo
https://orcid.org/0000-0001-9191-1405
These authors contributed equally to this work.

Handling Editor : Han, Sang-Wook
Received April 29, 2019; Revised June 26, 2019; Accepted July 2, 2019.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The lipopolysaccharide (LPS) composed of lipid A, core, and O-antigen is the fundamental constituent of the outer membrane in gram-negative bacteria. This study was conducted to investigate the roles of LPS in Burkholderia glumae, the phytopathogen causing bacterial panicle blight and seedling rot in rice. To study the roles of the core oligosaccharide (OS) and the O-antigen region, mutant strains targeting the waaC and the wbiFGHI genes were generated. The LPS profile was greatly affected by disruption of the waaC gene and slight reductions were observed in the O-antigen region following wbiFGHI deletions. The results indicated that disruption in the core OS biosynthesis-related gene, waaC, was associated with increased sensitivity to environmental stress conditions including acidic, osmotic, saline, and detergent stress, and to polymyxin B. Moreover, significant impairment in the swimming and swarming motility and attenuation of bacterial virulence to rice were also observed in the waaC-defective mutant. The motility and virulence of O-antigen mutants defective in any gene of the wbiFGHI operon, were not significantly different from the wild-type except in slight decrease in swimming and swarming motility with wbiH deletion. Altogether, the results of present study indicated that the LPS, particularly the core OS region, is required for tolerance to environmental stress and full virulence in B. glumae. To our knowledge, this is the first functional study of LPS in a plant pathogenic Burkholderia sp. and presents a step forward toward full understanding of B. glumae pathogenesis.
Keywords : Burkholderia glumae, lipopolysaccharide, stress tolerance, virulence


October 2019, 35 (5)
  • DOAJ
  • ORCID