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Identification of Differentially Up-regulated Genes in Apple with White Rot Disease
Plant Pathol. J. 2019;35:530-537
Published online October 1, 2019
© 2019 The Korean Society of Plant Pathology.

Yeo-Jin Kang1, Young Koung Lee2,3, and In-Jung Kim1*

1Faculty of Biotechnology, College of Applied Life Sciences, SARI, Jeju National University, Jeju 63243, Korea
2Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
3Division of Biological Sciences and Institute for Basic Science, Wonkwang University, Iksan 54538, Korea
Correspondence to: Phone) +82-64-754-3357, FAX) +82-64-756-3351
E-mail) ijkim@jejunu.ac.kr
ORCID
In-Jung Kim
https://orcid.org/0000-0003-4554-4872

Handling Editor : Jeon, Junhyun
Received October 9, 2018; Revised May 10, 2019; Accepted June 27, 2019.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Fuji, a major apple cultivar in Korea, is susceptible to white rot. Apple white rot disease appears on the stem and fruit; the development of which deteriorates fruit quality, resulting in decreases in farmers' income. Thus, it is necessary to characterize molecular markers related to apple white rot resistance. In this study, we screened for differentially expressed genes between uninfected apple fruits and those infected with Botryosphaeria dothidea, the fungal pathogen that causes white rot. Antimicrobial tests suggest that a gene expression involved in the synthesis of the substance inhibiting the growth of B. dothidea in apples was induced by pathogen infection. We identified seven transcripts induced by the infection. The seven transcripts were homologous to genes encoding a flavonoid glucosyltransferase, a metallothionein-like protein, a senescence-induced protein, a chitinase, a wound-induced protein, and proteins of unknown function. These genes have functions related to responses to environmental stresses, including pathogen infections. Our results can be useful for the development of molecular markers for early detection of the disease or for use in breeding white rot-resistant cultivars.
Keywords : apple, Botryosphaeria dothidea, differentially expressed genes


October 2019, 35 (5)
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