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Full-Length Infectious Clones of Two New Isolates of Tomato Mosaic Virus Induce Distinct Symptoms Associated with Two Differential Amino Acid Residues in 128-kDa Protein
Plant Pathol. J. 2019;35:538-542
Published online October 1, 2019
© 2019 The Korean Society of Plant Pathology.

Go-Woon Choi1†, June-Pyo Oh1†, In-Sook Cho2†, Hye-Kyoung Ju1, Wen-Xing Hu1, Boram Kim1, Eun-Young Seo1, Jong-Seok Park3, Leslie L Domier4, John Hammond5* , Kihak Song6*, and Hyoun-Sub Lim1*

1Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Korea
2Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, RDA, Wanju 55365, Korea
3Department of Horticulture, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Korea
4Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA
5United States Department of Agriculture-Agricultural Research Service, Floral and Nursery Plants Research Unit, Beltsville, MD 20705, USA
6Department of Urology, Chungnam National University School of Medicine, Daejeon 34134, Korea
Correspondence to: John Hammond
Phone) +1-301-504-5313, FAX) +1-301-504-5096
E-mail) john.hammond@ars.usda.gov
ORCID
https://orcid.org/0000-0001-7593-747X
Kihak Song
Phone) +82-42-280-7777, FAX) +82-42-823-8679 E-mail) songkihak62@gmail.com
Hyoun-Sub Lim
Tel) +82-42-821-5766, FAX) +82-42-823-8679
E-mail) hyounlim@cnu.ac.kr
These authors contributed equally to this work.

Handling Editor : Yoon, Ju-Yeon
Received December 10, 2018; Revised June 2, 2019; Accepted June 10, 2019.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
In 2017, two new tomato mosaic virus (ToMV) isolates were collected from greenhouses in Buyeo, Chungcheongnam-do, South Korea. Full-length cDNAs of the new ToMV isolates were cloned into dual cauliflower mosaic virus 35S and T7 promoter-driven vectors, sequenced and their pathogenicities investigated. The nucleotide sequences of isolates GW1 (MH507165) and GW2 (MH507166) were 99% identical, resulting in only two amino acid differences in nonconserved region II and the helicase domain, Ile668Thr and Val834Ile. The two isolates were most closely related to a ToMV isolate from Taiwan (KJ207374). Isolate GW1 (Ile668, Val834) induced a systemic hypersensitive response in Nicotiana benthamiana compared with the isolate GW2, which a single residue substitution showed was due to Val834.
Keywords : difference of infectivity, full-length infectious cDNA clone, tobamovirus, tomato mosaic virus (ToMV)


October 2019, 35 (5)
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