Subcellular Responses in Nonhost Plant Infected with Pathogenic and Nonpathogenic Strains of Xanthomonas axonopodis pv.glycines

Jeon, Yong Ho; Kim, Jung Gun; Chang, Sung Pae; Hwang, In Gyu; Kim, Young Ho

The Plant Pathology Journal 2002;18(3):115-120.
Published online June 30, 2002.

Subcellular Responses in Nonhost Plant Infected with Pathogenic and Nonpathogenic Strains of Xanthomonas axonopodis pv.glycines

Yong Ho Jeon, Jung Gun Kim, Sung Pae Chang, In Gyu Hwang, Young Ho Kim

Abstract

Xanthomonas axonopodis pv. glycines, the causal agent of bacterial pustule of soybean, induces hypersensitive response (HR) in a non-host plant, hot pepper (Capsicum annuum). A wild-type strain (8ra) and its non-pathogenic mutant (8-13) of X. axonopodis pv. glycines were inoculated into the pepper leaf tissues and their subcellular responses to the bacterial infections were examined by electron microscopy. Ultrastructural changes related to HR were found in the leaf tissues infected with 8ra from 8 h after inoculation, characterized by separation of plasmalemma from the cell wall, formation of small vacuoles and vesicles, formation of cell wall apposition, and cellular necrosis. No such responses were observed in the tissues infected with the mutant. In 8ra, the bacterial cells were attached to the cell walls, with the cell wall material dissolved into and appearing to encapsulate the bacterial cells. The bacterial cells later became entirely embedded in the cell wall material. On the other hand, in 8-13, the bacterial cells were usually not attached tightly to the plant cell wall, and no or poor encapsulation of the bacteria by the wall material occurred, although these were encircled by rather loose wall materials at the later stages.

Key Words: cell death, electron microscopy, hot pepper, hypersensitive response, Xanthomonas axonopodis pv. glycines