Characterization and RT-PCR Detection of dsRNA Mycoviruses from the Oyster Mushroom, Pleurotus ostreatus

Seo, Jang Kyun; Lim, Won Seok; Jeong, Ji Hye; Yoo, Young Bok; Yie, Se Won; Kim, Kook Hyung

doi:2004.20.3.200

Plant Pathol J > Volume 20(3); 2004 > Article

The Plant Pathology Journal 2004;20(3):200-205.
DOI: https://doi.org/10.5423/PPJ.2004.20.3.200 Published online September 30, 2004.

Characterization and RT-PCR Detection of dsRNA Mycoviruses from the Oyster Mushroom, Pleurotus ostreatus

Jang Kyun Seo, Won Seok Lim, Ji Hye Jeong, Young Bok Yoo, Se Won Yie, Kook Hyung Kim

Abstract

The partial nucleotide sequences of the genomic dsRNA mycovirus infecting Pleurotus ostreatus isolates ASI2223 and Suhan were determined and compared with those of mycoviruses belonging to partitiviruses and totiviruses. Partial nucleotide sequences of the purified dsRNA from ASI2223 and Suhan showed RNA-dependent RNA polymerase sequences that are closely related to those of partitiviruses, including Fusarium poae virus 1, Fusarium solani virus, Rhizoctonia solani virus, Discula destructiva virus 2, and Oyster mushroom isometric virus 2. Specific primers were designed for RT-PCR detection of dsRNA viruses from the P. ostreatus isolate ASI2223 and Suhan. Two virus specific primer sets were found to specifically detect each virus among six sets of designed oligonucleotide primers. Collectively, these results suggest that dsRNA mycoviruses from P. ostreatus isolates ASI2223 and Suhan belong to the family Partitiviridae, although, they are not the same virus species. Our results also suggest that these virus-specific primer sets can be employed for the specific detection of each viral sequence in infected tissues.

Key Words: dsRNA mycovirus, partitivirus, Pleurotus ostreatus, RT-PCR detection