Mixed Infection of 16S rDNA I and V Groups of Phytoplasma in a Single Jujube Tree

Lee, Sang Hun; Han, Sang Sub; Cha, Byeong Jin

doi:2009.25.1.21

Plant Pathol J > Volume 25(1); 2009 > Article

The Plant Pathology Journal 2009;25(1):21-25.
DOI: https://doi.org/10.5423/PPJ.2009.25.1.021 Published online March 31, 2009.

Mixed Infection of 16S rDNA I and V Groups of Phytoplasma in a Single Jujube Tree

Sang Hun Lee, Sang Sub Han, Byeong Jin Cha

Abstract

Jujube trees infected with phytoplasma exhibit symptoms of typical witches` broom, such as yellowing, abnormally small leaves, short internodes and proliferation of shoots. A 1.2 kb fragment of the 16S rDNA from jujube phytoplasma was generated by R16F2n/ R16R2 primer pair from earlier amplified P1/P7 PCR products of cloned jujube witches` broom phytoplasmas. Enzymatic restriction fragment length polymorphism (RFLP) and sequence analysis of 16S rDNA revealed that the jujube tree was infected with 16S rDNA I and V groups of phytoplasmas. Extensive comparative analyses of restriction enzyme profiles from Alu I, Hha I, Msp I, and Rsa I clearly classified the two into different phytoplasma groups. The phylogenic analyses based on 16S rDNA showed that the similarity of the two different clones was 87.5%. This is the first report of a mixed phytoplasmal infection in a single jujube tree.

Key Words: 16S rDNA, jujube witches broom, mulberry dwarf phytoplasma, phytoplasma, RFLP