RT-PCR Detection of Five Quarantine Plant RNA Viruses Belonging to Potyand Tospoviruses

Lee, Jong Seung; Cho, Won Kyong; Choi, Hong Soo; Kim, Kook Hyung

doi:2011.27.3.291

Plant Pathol J > Volume 27(3); 2011 > Article

The Plant Pathology Journal 2011;27(3):291-296.
DOI: https://doi.org/10.5423/PPJ.2011.27.3.291 Published online September 30, 2011.

RT-PCR Detection of Five Quarantine Plant RNA Viruses Belonging to Potyand Tospoviruses

Jong Seung Lee, Won Kyong Cho, Hong Soo Choi, Kook Hyung Kim

Abstract

In order to detect quarantine plant viruses, we developed reverse transcription-polymerase chain reaction (RT-PCR) primer pairs for five single-stranded (ss) plant RNA viruses that are not currently reported in Korea but could be potential harmful plant viral pathogens. Three viruses such as Chilli veinal mottle virus (ChiVMV), Colombian datura virus (CDV), and Tobacco etch virus (TEV) belong to the genus Potyvirus while Chrysanthemum stem necrosis virus (CSNV) and Iris yellow spot virus (IYSV) are members of the genus Tospovirus. To design RT-PCR primers, we used reported gene sequences corresponding to the capsid protein and polyprotein for ChiVMV, CDV, and TEV while using nucleocapsid protein regions for CSNV and IYSV. At least two different primer pairs were designed for each virus. Fifteen out of 16 primer pairs were successfully applied in detection of individual quarantine virus with high specificity and efficiency. Taken together, this study provides a rapid and useful protocol for detection of five quarantine viruses.

Key Words: Potyvirus, Quarantine, RT-PCR, ssRNA virus, Tospvirus