| Identification of Luteovirus Nucleotide Sequences in Mild Yellow-Edge Diseased Strawberry Plants |
|
Magdy Shaban Montasser, Husain Al Awadhi, Ahmed Hadidi |
|
|
| Abstract |
|
The availability of nucleotide sequences of coat protein gene of Potato leafroll virus (PLRV) permitted the construction of DNA primers that were utilized for cDNA synthesis. Polymerase chain reaction (PCR) products of a 487 bp. and approximately 500 bp DNA fragments were amplified from nucleic acid extracts of PLRV-infected tissue and strawberry mild yellow-edge (SMYE) diseased strawberry tissue, respectively. The amplified DNA fragments were further differentiated by hybridization analysis with a cDNA probe for the coat protein gene of PLRV and restriction fragment length polymorphism (RFLP) analysis. These results suggest that a luteovirus is associated with the SMYE disease. |
| Key Words:
Amplification, Luteovirus, PLRV, RFLP, SMYEV, strawberry |
|
PDF Links
Full text via DOI
Download Citation
