First Report of Tobacco mild green mosaic virus Infecting Pepper in Korea

Choi, Gug Seoun; Kim, Jae Hyun; Ryu, Ki Hyun; Choi, Jang Kyung; Chae, Soo Young; Kim, Jeong Soo; Chung, Bong Nam; Kim, Hyun Ran; Choi, Yong Mun

doi:2002.18.6.323

Plant Pathol J > Volume 18(6); 2002 > Article

The Plant Pathology Journal 2002;18(6):323-327.
DOI: https://doi.org/10.5423/PPJ.2002.18.6.323 Published online December 31, 2002.

First Report of Tobacco mild green mosaic virus Infecting Pepper in Korea

Gug Seoun Choi, Jae Hyun Kim, Ki Hyun Ryu, Jang Kyung Choi, Soo Young Chae, Jeong Soo Kim, Bong Nam Chung, Hyun Ran Kim, Yong Mun Choi

Abstract

A rod-shaped virus was isolated from pepper showing mild mosic during the winter growing seasons of 2001 and 2002 in Korea. Based on its biological reactions, serological relationships, reverse transcription-poly-merase chain reaction (RT-PCR) using specific primers, and nucleotide sequence analysis of coat protein (CP) gene, the isolated virus was identified as Tobacco mild green mosaic virus (TMGMV) and designated as Korean pepper isolate (TMGMV-KP). Crude sap from infected tissue was mechanically transmitted to various indicator plants, which produced characteristic symptoms of tobamovirus infection. However, no symptom was observed in Gomphorena globosa. In RT-PCR assays with specific primers for respective detection of TMGMV, Tobacco mosaic virus (TMV), Pepper mild mottle virus (PMMoV), and Tomato mosaic virus (ToMV), a single strong band of about 500 bp in length was produced from the sample used only with TMGMV primers. The amplified DNA was cloned and the nucleotide sequence was determined. Sequence comparisons with the CP gene of other tobamoviruses indicated that TMGMV-KP shared 99.3% identity with TMGMV Japanese isolate and only 59.1, 58.6, and 58.1% identity with TMV, PMMoV and ToMV, respectively. This is the first report of TMGMV in Korea.

Key Words: identification, pepper, sequence, Tobacco mild green mosaic virus, Tobamovirus