Indentification of Differentially Displayed Genes of a Pseudomonas Resistant Soybean(Glycine max)

Kang, Sang Gu; Cha, Hyeon Wook; Chang, Moo Ung; Park, Eui Ho

The Plant Pathology Journal 2003;19(5):239-247.
Published online October 31, 2003.

Indentification of Differentially Displayed Genes of a Pseudomonas Resistant Soybean(Glycine max)

Sang Gu Kang, Hyeon Wook Cha, Moo Ung Chang, Eui Ho Park

Abstract

In Korea, a local soybean (Glycine max) genotype 561 was found to be strongly resistant to a virulent bacterial strain of a Pseudomonas sp. SN239. Specific genes involved in the resistance of the soybean genotype 561 were identified and the pattern of gene expression against the Pseudomonas infection was analyzed using differential-display reverse transcription PCR (DDRTPCR). More than 126 cDNA fragments representing mRNAs were induced within 48 hours of bacteria inoculation. Among them, 28 cDNA fragments were cloned and sequenced. Twelve differentially displayed clones with open reading frames had unknown functions. Sixteen selected cDNA clones were homologous to known genes of other organisms. Some of the identified cDNAs were pathogenesis-related (PR) genes and PRlike genes. These cDNAs included a putative calmodulinbinding protein; an endo-1,3-1,4-β-D-glucanase; a β-1,3-endoglucanase; a β-1,3-exoglucanase; a phytochelatin synthetase-like gene; a thiol protease; a cycloartenol synthase; and a putative receptor-like serine/threonine protein kinase. Among them, four genes were found to be putative PR genes induced significantly by the Pseudomonas infection. These included a calmodulinbinding protein gene, a β-1,3-endoglucanase gene, a receptor-like serine/threonine protein kinase gene, and pS321 (unknown function). These results suggest that the differentially expressed genes may mediate the strong resistance of soybean 561 to the strain SN239 of Pseudomonas sp.

Key Words: Differential display, disease resistant soybean, gene expression, Glycine max, pathogenesis-related (PR) genes, Pseudomonas