Virus Resistant and Susceptible Transgenic Nicotiana benthamiana Plants Expressing Coat Protein Gene of Zucchini green mottle mosaic virus for LMO Safety Assessment

Kim, Min Jea; Choi, Sun Hee; Kim, Tae Sung; Park, Min Hye; Lim, Hee Rae; Oh, Kyung Hee; Kim, Tae San; Lee, Min Hyo; Ryu, Ki Hyun

doi:2004.20.3.206

Plant Pathol J > Volume 20(3); 2004 > Article

The Plant Pathology Journal 2004;20(3):206-211.
DOI: https://doi.org/10.5423/PPJ.2004.20.3.206 Published online September 30, 2004.

Virus Resistant and Susceptible Transgenic Nicotiana benthamiana Plants Expressing Coat Protein Gene of Zucchini green mottle mosaic virus for LMO Safety Assessment

Min Jea Kim, Sun Hee Choi, Tae Sung Kim, Min Hye Park, Hee Rae Lim, Kyung Hee Oh, Tae San Kim, Min Hyo Lee, Ki Hyun Ryu

Abstract

Transgenic Nicotiana benthamiana plants harboring coat protein (CP) gene of Zucchini green mottle mosaic virus (ZGMMV) were generated for virus-resistant screening and complementation analysis of related viruses for environmental safety assessment (SA) of living modified organism (LMO) purposes. Transformation of leaf disc of N. benthamiana was performed by using Agrobacterium-mediated method and the pZGCPPGA748 containing the ZGMMV CP and NPTII genes. Two kinds of transgenic homozygous groups, virus-resistant and virus-susceptible N. benthamiana lines, were obtained by screening of challenging homologous virus for T1 generations. These two pathologically different lines can be useful for host-virus interactions and LMO environmental SA.

Key Words: Living modified organism, LMO, Tobamovirus, transformation, transgenic plant, Zucchini green mottle mosaic virus