Plant Pathol J > Volume 20(4); 2004 > Article
The Plant Pathology Journal 2004;20(4):302-307.
DOI:    Published online December 31, 2004.
Development of Molecular Detection of Three Species of Seed-Transmissible Viruses Useful for Plant Quarantine
Bo Young Lee, Hee Rae Lim, Ji Yong Choi, Ki Hyun Ryu
Three pairs of specific primers were developed for rapid and precise RT-PCR detection of three seedtransmissible viruses, namely Peanut clump virus (PCV, Pecluvirus), White clover mosaic virus (WCIMV, Potexvirus) and Carrot red leaf virus (CaRLV, Luteovirus). Each primer set was found in conserved region through multiple sequence alignment in the DNAMAN. Total nucleic acids extracted from PCV-, WCMV-, and CaRLV-infected seeds and healthy plants were used for RT-PCR detection using each virus-specific primer. Sizes of PCV, WCIMV, and CaRLV PCR products were 617bp (PCV-uni5 and PCV-uni3 primers), 561bp (WCMV-CP5 and WCMV-CP3 primers), and 626bp (CL1-UP and CL2-DN primers); which corresponded to the target sizes. Nucleotides sequences of each amplified cDNA were confirmed which belonged to the original virus. This study suggests that these virusspecific primer sets can specifically amplify viral sequences in infected seeds. Thus, they can be used for specific detection of three viruses (PCV, WCMV and CaRLV) from imported seed samples for plant quarantine service.
Key Words: Carrot red Leaf virus, CaRLV, detection, Peanut clump virus, PCV, plant quarantine, RT-PCR

Editorial Office
Rm,904 (New Bldg.) The Korean Science & Technology Center 22,
Teheran-ro 7-Gil, Gangnamgu, Seoul 06130, Korea
Tel: +82-2-557-9360    Fax: +82-2-557-9361    E-mail:                

Copyright © 2024 by Korean Society of Plant Pathology.

Developed in M2PI

Close layer
prev next