Cloning and Sequencing of Coat Protein Gene of the Korean Isolate of Rice stripe virus

Lee, Bong Choon; Hong, Yeon Kyu; Kwak, Do Yeon; Park, Sung Tae; Choi, Jo Im; Lee, Key Woon

doi:2004.20.4.313

Plant Pathol J > Volume 20(4); 2004 > Article

The Plant Pathology Journal 2004;20(4):313-315.
DOI: https://doi.org/10.5423/PPJ.2004.20.4.313 Published online December 31, 2004.

Cloning and Sequencing of Coat Protein Gene of the Korean Isolate of Rice stripe virus

Bong Choon Lee, Yeon Kyu Hong, Do Yeon Kwak, Sung Tae Park, Jo Im Choi, Key Woon Lee

Abstract

The coat protein gene of Korean isolate of Rice stripe virus (RSV-Kr) was cloned and its nucleotide sequence was determined. Total RNA was extracted from infected leaves and RSV viral RNA was detected by using RT-PCR with specific primer of coat protein gene. The result of RT-PCR showed a specific band. Purified RT-PCR products of coat protein gene were ligated into the pGEM-T Easy plasmid vector and cloned cDNA was obtained for nucleotide sequence determination. Coat protein gene of RSV-Kr consisted of 969 bp long encoding a protein of 322 amino acids. RSV-Kr showed 94%-99% sequence identities to that of Japanese- and Chinese isolates.

Key Words: Coat protein, Rice stripe virus, sequence