Characterization of Trichoderma spp. Associated with Green Mold of Oyster Mushroom by PCR-RFLP and Sequence Analysis of ITS Regions of rDNA

Park, Myung Soo; Seo, Geon Sik; Bae, Kyung Sook; Yu, Seung Hun

doi:2005.21.3.229

Plant Pathol J > Volume 21(3); 2005 > Article

The Plant Pathology Journal 2005;21(3):229-236.
DOI: https://doi.org/10.5423/PPJ.2005.21.3.229 Published online September 30, 2005.

Characterization of Trichoderma spp. Associated with Green Mold of Oyster Mushroom by PCR-RFLP and Sequence Analysis of ITS Regions of rDNA

Myung Soo Park, Geon Sik Seo, Kyung Sook Bae, Seung Hun Yu

Abstract

Molecular profiles of PCR-RFLP and sequence analysis of internal transcribed spacer (ITS) regions of rDNA were compared between morphologically distinguishable species of Trichoderma isolated from substrates of oyster mushroom in Korea, T. atroviride, T. citrinoviride,T. harzianum, T. longibrachiatum, T. virens, and two unidentified species, Trichoderma sp. 1 and 2. PCRRFLP analysis divided the Trichoderma spp. into six RFLP groups, A, B, C, D, E, and F. The RFLP groups were generally agreed with described morphological species, except that the RFLP group A containing the two unidentified species. A neighbor-joining tree based on ITS sequences well supported RFLP groups observed by RFLP analysis of ITS regions of rDNA.Additionally, the two unidentified species, Trichoderma sp. 1 and 2, which could not be distinguished by PCRRFLP analysis, were separated in sequence analysis of ITS regions of rDNA.

Key Words: ITS regions of rDNA, oyster mushroom, PCR-RFLP, Trichoderma