Near-Isogenic Lines for Genes Conferring Hypersensitive Resistance to BacterialSpot in Chili Pepper

Kim, Byung Soo; Kim, Young Chun; Shin, Kwang Sik; Kim, Jeong Hoon

The Plant Pathology Journal 2007;23(3):155-160.
Published online September 30, 2007.

Near-Isogenic Lines for Genes Conferring Hypersensitive Resistance to BacterialSpot in Chili Pepper

Byung Soo Kim, Young Chun Kim, Kwang Sik Shin, Jeong Hoon Kim

Abstract

In order to develop chili pepper bacterial spot resistant cultivars and near-isogenic lines (NILs) to prompt the molecular mapping of the resistance gene, we have run backcross breeding program since 1994. Two resistance genes against Xanthomonas axonopodis pv. vesicatoria Bs2 from Fla. XVR 3-25 and Bs3 from our breeding line 25-11-3-2, were introduced into a land race, Chilseongcho (abbreviated to Chilseong hereafter) with good fruit guality. We report here the testing of BC4F3 to BC4F5. We found that BC4F5 lines of the crosses were homozygous with respect to the respective genes of introduction. The lines, in which Bs2 gene was introduced, were hypersensitively resistant to both race 1 and race 3 of X. axonopodis pv. vesicatoria, whereas, those in which Bs3 was introduced were resistant to race 1.

Key Words: breeding, Chili pepper, resistance, Xanthomonas axonopodis pv. vesicatoria